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BX53
The new BX53 provides advanced modularity to suit a variety of observation styles. The rigid, compact space-saving frame features forward-mounted, easy-to-operate controls for improved efficiency and greater operator comfort. New operator controls are accessible from either side of the microscope, and an ECO sensor automatically turns off the transmitted light when the operator is not seated at the microscope. True to the Olympus philosophy, the BX53 offers an extended range of accessories and objectives to satisfy today’s most discerning microscopists.
The new BX53 offers outstanding features and performance
- Highly scalable microscope system to answer the demands of research today and tomorrow.
- New fluorescence illuminators, filter cubes and Olympus high performance filters provide added convenience to researchers – there’s no need for tools to add a filter cube to the new 8-position illuminator turret.
- Designed for maximum flexibility, the BX53 features forward-oriented controls for ease of access and improved operator comfort.
- 12V/100W halogen illumination source provides bright, balanced illumination for brightfield and contrast observation methods.
- Ergonomic Y-shaped frame for maximum stability and comfort, with low position focus control knobs located on both the left and right sides of the frame
- Olympus UIS2 optics deliver bright, sharp, high-contrast images.
- The new motorized 8-position universal condenser and encoded objective nosepiece are integrated with cellSens software to automatically change contrast inserts (i.e. DIC prisms or phase contrast annuli) when the magnification changes.
Flexible System Format
- Modular design accommodates a variety of accessories without any compromise to image quality.
- Accepts a variety of observation attachments for multiple viewers and digital cameras.
- 100W Halogen for transmitted or reflected light, 100W HBO* (Mercury) and 75W XBO (Xenon), LED, and 3rd party metal-halide* illumination options for reflected light and fluorescence.
- Ceramic-coated stage with left-hand or right-hand low drive control, rotating mechanism and torque adjustment mechanism, optional rubber grips (non-stick grooved coaxial, plain, rotatable stages and others are also available)
Supports a full range of observation methods
Fluorescence
The 8 position FluorescenceIlluminator allows flexible responses to various fluorescence specimens. Olympus applies an outstanding filter coating technology to all fluorescence mirror units in order to produces high transmissions, sharp cut-offs and efficient detections of fluorescence.
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Breast tissue
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Brightfield
BX53 is equipped with a gradation filter improving neighboring light quantity in the transmission illumination system by default.
Even microscopy is the photography with the digital camera, but an evenly uniform observation image is provided to the field of vision outskirts.
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Stomach tissue
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Phase contrast
Universal phase-contrast objectives have high transmission, producing well-balanced images with high contrast.
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Muscle
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Differential interference contrast(DIC)
High contrast can be obtained even in high magnification observations of thin specimens, such as culture cells. The method enables observations with high resolution but less glare even for thick specimens used in developmental and genetic research, such as finely-tructured diatoms.
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Brain section of mouse at embryonic day 15
Image is courtesy of Fumio Matsuzaki, Ph.D., Daijiro Konno, Ph.D.
Laboratory for Cell Asymmetry
RIKEN Center for Developmental Biology
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Polarizing Observation
There are intermediate attachments (U-OPA/U-CPA) for orthoscopic and orthoscopic/conoscopic viewing.Various compensators make it possible to observe a wide range of retardation.
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Amyloid
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Darkfield
Two darkfield condensers are provided: dry condenser with magnification range from 10× to 100× and oil immersion condenser with magnification range from 20× to 100× for darkfield contrast. Universal condensers also allow simple darkfield contrast.
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Spirogyra
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Customizable Control Layout
Light intensity now can be controlled with the dial in front, and transmitted filters and fluorescence shutters are operable from either side. Detachable fine focus handle can be attached on either side of the scope based on operator preference. The BX53 frees the operator to create your own working environment with the microscope.
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Frontal operation for ease of use
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Observation Tubes Lineup Caters to Your Individual Needs
Tilting trinocular tube/ U-TTR-2 enables to accommodate any operator posture with the slant angle of binocular from 5 through 35 degrees. In addition, Olympus offers versatile types of tubes.
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Tilting trinocular tube
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Automatic Switching
Switching objectives on the motorized 7-position revolving nosepiece, integrated with the motorized universal condenser, enables an automatic switch to the optimal prism. Simplified prism switches accelerate observations.
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An electric condenser and an electric revolver corresponding to the observation method.
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Energy saving Switch Turns off Automatically
The motion sensor detects when an operator leaves and automatically turns off the transmitted light lamp after around 30 minutes.
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Energy saving switch
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High S/N fluorescence imaging
Latest Coating Technology and Stary Light Reduction
Olympus applies an outstanding filter coating technology to all fluorescence mirror units in order to produce high transmissions, sharp cut-offs and efficient detections of fluorescence. The low reflection function eliminates over 99% of stray light, producing a high S/N ratio.
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Newly designed mirror units
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High Transmission Objectives with Reduced Autofluorescence
Olympus UIS2 objectives are made with superior low-autofluorescence glass, anti-reflection coats and lens joining materials, improving the S/N ratio. Effective detections of subtle fluorescence emissions even with weak excitation light deliver ideal performance in fluorescence imaging.
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High Transmission Objectives with
Reduced Autofluorescence
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High Fluorescence Performance in imaging
Fluorescence illuminators BX3-RFAA and BX3-RFAS equipped with a Fly's-eye lens provide uniform illumination. Even, uniform fluorescence illumination facilitates postimaging enhancements and processing. Furthermore, mirror units can easily be replaced.
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No tools are required for replacement.
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| | Out of Alignment | Alignment |
| Conventional illuminator |
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| BX3-RFAS |
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Hand switch for exposure
The U-HSEXP hand switch for exposure attaches easily on the microscope, and allows the operator to capture an image without having to take his/her eyes away from the specimen. The exposure switch is located close to the focus handle creating minimal movement during operation.
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Hand switch for exposure
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cellSens Imaging Software
A Single Software Solution for Smooth Experimental Workflow from Start to Finish cellSens® is available in three different versions to suit individual requirements.
Dimension - comprehensive report-making capabilities streamlines operations from beginning to end. Also available in a desktop version for offline analysis.
Standard - meets the needs for basic image acquisition and analysis while simplifying operation Entry - simplest package for snapping an image
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| Microscope frame | Optical system |
UIS2 optical system |
| Focus |
Vertical stage movement: 25 mm stage stroke with coarse adjustment limit stopper, Torque adjustment for coarse adjustment knobs, Stage mounting position variable, High sensitivity fine focusing knob (minimum adjustment gradations: 1 µm) |
| Illuminator |
Built-in Koehler illumination for transmitted light, Light preset switch, Light intensity LED indicator, Built-in filters (LBD-IF, ND6, ND25, optional) 12 V 100 W halogen bulb (pre-centered) |
| Revolving nosepiece |
Interchangeable reversed quintuple/coded quintuple/sextuple/septuple/coded septuple nosepiece |
| Observation tube | Widefield (F.N. 22) |
• Widefield tilting trinocular
• Widefield trinocular
• Widefield tilting binocular
• Widefield tilting,Telescopic,Lifting binocular
• Widefield ergo binocular
• Widefield binocular |
| Super widefield (F.N. 26.5) |
• Super widefield trinocular
• Super widefield erect image trinocular |
| Stage |
Ceramic-coated coaxial stage with left or right hand low drive control: with rotating mechanism and torque adjustment mechanism, optional rubber grips available (Non stick grooved coaxial, plain, rotatable stages are also available) |
| Condenser |
• Abbe (N.A. 1.1), for 4×-100×
• Swing out Achromatic (N.A. 0.9), for 1.25×-100× (swing-out: 1.25×-4×)
• Achromatic Aplanatic (N.A. 1.4), for 10×-100×
• Phase contrast, darkfield (N.A. 1.1), [phase contrast: for 10×-100×, darkfield: for 10×-100× (up to N.A. 0.80)]
• Universal (N.A. 0.9), for 1.25×-100× [swing-out: 1.25×-4×, with oil top lens:(N.A. 1.4)]
• Low (N.A. 0.75), for 2×-100× (Dry)
• Ultra low (N.A. 0.16), for 1.25×-4×
• Darkfield dry (N.A. 0.8-0.92), for 10×-100×
• Darkfield oil (N.A. 1.20-1.40), for 10×-100× |
| Fluorescence illuminator |
• Multi-purpose coded type (F.N. 22, 8-position mirror unit turret, 4-position ND slider)
• Economical type (F.N. 26.5, 8-position mirror unit turret) |
| Fluorescence light source |
100 W Hg apo lamp housing and transformer, 100 W Hg lamp housing and transformer or 75 W Xe lamp housing and transformer |
| Operating environment |
• Indoor use
• Ambient temperature : 5 ° to 40 °C (41 ° to 104 °F)
• Maximum relative humidity : 80 % for temperatures up to 31° C (88 °F), decreasing linearly through 70 % at 34 °C(93 °F), 60 % at 37 °C (99 °F), to 50 % relative humidity at 40 °C (104 °F)
• Supply voltage fluctuations : Not to exceed ±10 % of the normal voltage |
*The control box for motorized units is designed for the BX3 use in industrial environments for the EMC performance.(IEC61326-1 Class A device)
Using it in a residential environment may affect other equipment in the environment.
BX53 dimensions (unit: mm)
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