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FV10i

Just as the digital camera completely changed the concept of "Photography", now, Olympus FV10i will change the world of the confocal laser scanning microscope. The world's first self-contained Confocal Laser Scanning Microscope.

FV10i is the world's first self-contained Confocal Laser Scanning Microscope.

The unique advantage of the FV10i is its self-contained design. We have completely re-engineered the design of the confocal laser scanning microscope into a self-contained package integrated with a variety of functions including an incubator and a laser combiner. You can install the FV10i easily in a laboratory without having to prepare a dedicated room. The FV10i also has unique features like a vibration isolation function, and light-tight cover, eliminating the need for a dark room. The FV10i has the same functionality of a high end confocal laser scanning microscope with easy-to-use software delivering it in a compact design.

Installed anywhere, and used by anyone.

The biggest advantage of FV10i is unique self-contained design

FV10i inner look

Dark room free

The Microscope body and light tight cover are integrally combined. You can use the FV10i with ease in a laboratory, unlike conventional confocal laser scanning microscopes which require a dark room.

Microscope function

The FV10i’s excellent optical and mechanical modules are totally integrated. The FV10i can capture images from 10× to 600× magnification with 10×, 60× objectives and optical zoom.

Scanning unit

The system is equipped with a detector which automatically sets conditions in accordance with fluorescence dye on a scanning unit.

Vibration isolation function

Equipped with built-in vibration insulators. A vibration isolation table is not required. You can install it directly on your experimental table.

Laser combiner

Equipped with four diode laser units, each unit utilizing a compact diode laser of longer life and power-savings.

Stress-free operation for every user.

Place a specimen on the stage and close the cover. These two steps complete the work of the user.

Acquisition is made easy using the FV10i software interface

Sample setting

Place a specimen, and select a fluorescence dye. The FV10i automatically selects the most suitable imaging conditions based on the fluorescence dye selection.

software view

Image mapping menu

Just click a <Start> button, and a map image of the specimen is created automatically. Users can easily identify the point he or she wants to capture.

software2

Image capturing

Through the sophisticated operating software, the image capture area or zoom magnification can be set quickly and then click of a button to complete image capturing.

software3

Image capturing with use of sophisticated menus.

No experience is required with the FV10i even for sophisticated confocal imaging. The navigation function leads a first-time user to operate the FV10i perfectly.

software4

Mapping area selection

The area is displayed according to the type of specimen holder used, such as a 35mm dia. dish or glass slide. You can also change of the area with just a single click operation.

Observation mode selection

Five types of observation modes can be selected including time-lapse, Z-stack, and multi-area.

Control screen

Imaging conditions can be set in detail with operation of various controllers.

Navigation function

Clicking the <Start> button in the Navigation function shows the operational procedure and highlights the operational button. Just follow the navigational guidance to easily complete your imaging.

Navigation function

Various observation mode / editing-analysis software

Five types of observation modes can be selected. The image you captured can perform effective editing-analysis by attached software.

	Z-stack In Z-stack mode, images are repeatedly acquired in different focus positions. Three-dimensional images can constructed.
	Multi-area - Z-stack - time-lapse The imaging where all three functions are performed.
	Time-lapse In time-lapse mode, images are continuously acquired at predetermined intervals.
	Z-stack - time-lapse The imaging which integrates Z-stack and time- lapse is possible.
	Multi-area - Z-stack - time-lapse The imaging where all three functions are performed.

Editing / Analysis software

Olympus original software for editing and analysis is provided as part of the standard specifications.
You can edit and analyze images taken by FV10i in various ways.

Line up

The FV10i offers two types of products with superior performance and function in a self-contained design.

FV10i-O Oil-based Model -

focusing on basic functions

The best oil immersion phase 60× objective, with a numerical aperture of 1.35 enabling high-quality imaging. Olympus have a low auto-fluorescence immersion oil. more

Full image

FV10i-W Water-based Model -

optimal for live cell time-lapse imaging.

The system is equipped with water-immersion objectives which are optimally suited for time-lapse imaging of live cells with a simplified built-in incubator.

Full image

<FV10i-W feature>

Simplified built-in incubator
The environment in the culture chamber is maintained at temperature - 37 degrees Celsius, Humidity of - 90%, and CO² concentration of - 5%^*.

* When using CO² 5% cylinder

Water is automatically supplied to the water-immersion objective
The newly developed automatic water dispensing system enables the FV10i to supply water to the top of the water-immersion objective. You can continue long term time-lapse imaging without worrying about insufficient immersion media, Water is supplied automatically when the objective is moved into the observation position.

Detection of cover glass thickness and automatic adjustment of the correction collar
The system is equipped with the capability to detect the thickness of the cover glass, allowing it to adjust correction collar automatically, when using the water-immersion objective. This assures imaging is performed each time with optimal conditions.

The advanced optical performance and feature-rich functions to support.

FV10i support to pursue high-definition confocal images and efficient stress-free imaging.

The system is equipped with 4 wavelength diode lasers.

The system is equipped with 4(405/473/559/635nm)lasers. Maintenance-free and power-saving diodes lasers with longer operating lives are employed in all the laser unit.

Detector utilizes a newly developed spectrum method.

The detecting mechanism has two fluorescence channels, and one phase contrast channel. The fluorescent channels use newly developed spectrum method comprising grating, beam splitter, and slit. In addition, they are equipped with the variable barrier filter function where the most suitable wavelength width is set automatically in accordance with the characteristics of the fluorescence dye.

Two sequential modes.

The FV10i is equipped with two sequential modes. You can acquire images through line sequence without crosstalk in imaging with two fluorescence dyes, and with three or four dyes in frame sequences with the virtual channel function.

Objectives of 10× and 60× are mounted on the system.

The system is equipped with objectives of 10× and 60×. Zoom magnification can be changed continually from 10× to 600×. The most suitable imaging area can be set depending on size of the specimen.

Equipped with specimen holders

The system is equipped with specimen holders, usable for a dia. 35mm glass bottom dishes, glass sliders, and cover glass chambers (8 wells type).

[W]=FV10i-W, [O]=FV10i-O

Laser light source	Ultraviolet / Visible light LD lasers:	405nm(18mW),473nm(12.5mW), 559nm(15mW),635nm(10mW)
Laser light source	Modulation:	Continuously Variable by the LD direct modulation (0.1%-100%, 0.1% inclement) Line return period - laser OFF
Scanning	Scanning method	2 galvanometer scanning mirrors
Scanning	Scanning mode	Pixel size: 256 × 256 - 1024 × 1024 Scanning speed: 1.1 s / frame (for pixel size 512 × 512, High Speed scanning mode) Focusing scanning: High frame rate scan by Y- direction interlace scanning (×1, ×2, ×4) Dimension: XYT, XYZ, XYZT Rotation scanning: 0-359.9° in 0.1° increments
Detection	Detector module	Fluorescence: 2 channels, Phase Contrast: 1 channel Variable barrier filter mechanism for fluorescence channel by diffraction grating and slit
	Detection method	Analog integration detection by Photomultiplier
	Pinhole	Single motorized pinhone Pinhole diameter: Ø50-800µm automatic setting (adjustable to ×1.0, ×1.5, ×2.0, and ×2.5)
	Field number	18
	Optical zoom	10× objectives: 1× — 6× in 0.1× increments 60× objectives: 1× — 10× in 0.1× increments
	Automatic Exposure	Automatic setting of the laser intensity and photomultiplier sensitivity to fluorescence intensity.
Focus	Z-drive	Motorized focus Minimum increment: 0.01µm
	Objectives	Exclusively designed 10× phase contrast objective / NA 0.4 (equivalent to UPLSAPO 10x) Exclusively designed 60× phase contrast water-immersion objective / NA 1.2 (equivalent to UPLSAPO 60× W) / with motorized correction collar[W] Exclusively designed 60× phase contrast oil-immersion objective / NA 1.35 (equivalent to UPLSAPO 60× O) [O]
	Automatic focus (AF)	Automatic detection of interface between specimen and cover glass by laser reflection light detection Automatic detection of cover glass thickness and automatic setting of motorized correction collar[W]
	Water supply[W]	Automatic water supply and air cleaning mechanism for 60× Water-immersion objective
	Oil supply[O]	Manual As supporting mechanism, automatic moving of XY stage to oil supply position when switching to 60x
XY stage	XY driving method	Motorized XY stage module by stepping motor Minimum increment: 0.3µm
XY stage	Specimen holder	Only the dedicated specimen holder can be mounted For three glass bottom dishes with 35mm diameter[W], For one set of cover glass chamber (8 wells type)[W] Culture pod (for a glass bottom dish with 35mm diameter) [W], For a glass slide For a glass bottom dish with 35mm diameter[O]
Incubator[W]	Room environment:[W]	Temperature: 37±1°C Humidity: more than 90% CO2 concentration: 5% (recommended), 1 - joint fitting (ø2mm) for exterior CO² adjustor
Incubator[W]	Heating method[W]	Non-contact heating by resistive heater mounted on frame section
Control device	Controller	Dedicated controller PC/AT-compatible OS: Windows Vista Business, 32 bit (English version), CPU: Intel Core2Duo 3.0GHz RAM: 2GB × 2, HDD: 320GB × 2, Special PCI-Express I/F board built-in, Media: DVD-Multi drive built-in
Control device	LCD monitor	24 inch LCD monitor × 1, WUXGA (1920×1200)
Main software feature	Image acquisition mode	Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi area Z-stack time-lapse (Multi Area XYZT)
	Specimen setting	Automatic setting for fluorescence channel and laser according to Dye selected from Dye list
	Map image acquisition	Automatic selection of map image of 3×3 - 9x9 fields according to 10× objective lens, and manual selection of map acquisition area.
	Multi area time-lapse	Automatic multi area time-lapse by motorized XY stage Setting for each registered point: Image size, scanning speed, cross talk reduction, pinhole diameter, rotation angle, galvano zoom, acquisition channel, laser power, PMT sensitivity, Z condition Maximum resister number: 10 items per one container Maximum interval time: one hour Maximum acquisition number of times: 3000 times per one point
	Image acquisition area	Area appointment: All area, clipping square area (minimum area: 96 × 96 pixels)
	Image display	Display by channel, overlapping display, image in progress review
	Cross talk reduction	Line sequential action (2 channel), or frame sequential action (3 channel and 4 channel)
	Acquisition image file type	OLYMPUS image format (OIF)
	"Image file type available for viewing"	OLYMPUS image format (OIF, OIB), Multi-TIFF format (8/16 bit grey scale, index color, 24/32/48 bit color), JPEG, BMP, TIFF
	Image editing	LUT: pseudo color setting, contrast adjustment, Comment: inputting graphic, text, scale etc., image extraction, combination
	3D image construction	3D display: AlphaBrend method, Maximum intensity projection method 3D animation display, free orientation of cross section display
	Image processing	Various types of image filter: Median, Enhanced Edge, etc. Calculations: inter-image, arithmetic and logical operation
	Image analysis	Area and perimeter measurement, time-lapse measurement, colocalization analysis
Room environment	Temperature	18-28°C
Room environment	Humidity	30-80% (non condensing)