Confocal Scanner Box, CellVoyager^TMCV1000, is an fully integrated desk-top imaging system equipped with precision environment control, and composed of a CSU unit, laser light sources and an EMCCD camera, This epoch-making system enables long time observation of most delicate life processes, such as the generation of iPS/ES cells or embryogenesis quite easily and without the necessity of dark room operation. The strong advantage of substantially reduced photo toxicity/bleaching by dual Nipkow disc scanning of the CSU differentiates the CV1000 from any other imaging boxes.

Microlens enhanced dual Nipkow disc scanning

Nipkow spinning disk containing about 20,000 pinholes and a second spinning disk containing the same number of microlens to focus excitation laser light into each corresponding pinhole are mechanically fixed with a motor, and very rapidly raster scan the field of view with about 1,000 laser beams when rotated. Multi-beam scanning with the CSU not only increases scanning speed, but also results in significantly lower photo bleaching and photo toxicity, because multiple excitation needs only a low level of laser power at the specimen to fully excite fluorescence.

More than 1,500 units of the CSU series are used as the de facto standard tool for live cell imaging, worldwide.

Automatic objective lens switching 

When you select a lens with a higher magnification, the objective lens switches automatically and the image is shown at that magnification. With just one click, you can record a magnified image of whatever region interests you. With the double-disk unit (optional), you can select the pinhole size that works best with your chosen magnification, for optimal imaging. With a single CV1000 system, you can observe thick and large samples such as a whole mount embryo or an organ slice as well as small and complex samples such as a neurite. 

New
25 um pinhole disk
(for low magnification)
20X oil

Standard
50 um pinhole disk
(for high magnification)
60X oil

Cell Voyager 1000 - All in One Imaging Unit

Compact all-in-one unit

No need for a dark room
→use the CV1000 right at your lab bench.

Reliable environmental control

Temperature is precisely controlled inside the stage incubator and measurement unit, keeping cells healthy for long periods.

Double-disk unit with two pinhole sizes (option)

Select the optimal pinhole size depending on the magnification.
A direct optical path setting is available for bright field imaging.

 Early stage mouse embryoiFixed cell, X40)
Clear image was obtained from 25μm pinhole

Sample Holders (options)

Select the attachment that best meets your requirements. Use an attachment together with the stage incubator (for either single or triple 35 mm dish) to keep cells healthy during time lapse imaging. Attachments for micro-plates with up to 96 wells are now available.

35mmDish

35mmDish

Slide glass

Microplate

Autofocus

Automatically focuses on the glass-side surface of the specimen. Changes the autofocus setting at any time, even while performing time lapse imaging.

User friendly Software

Map view: Efficient sample search function

X-Y map view images covering a wide image area are automatically generated for easy comprehension of sample distributions. The map view function lets you view specific image areas with the same ease of use as a Web based map search function.

Recorded data viewer

Allows you to easily confirm previously recorded images while capturing time lapse images.

Area view

In addition to being able to view individual recording fields, you can select area view to see all the fields; this facilitates quick comprehension of movements occurring over a wide area.

Field: a single recording field Area: a group of recording fields, including 1x1 fields

Time lapse settings

You can set either a single interval or multiple intervals at specific time points. The intervals and the number of recordings can be changed at any time while time lapse images are being captured.

Correction of imaging area throughout the experiment

You can correct image centering of each field during a time-lapse imaging experiment, no more loss of long-term data!

Capable of various condition setting within one experiment

Channel settings of laser power, exposure time, and EM gain can be set for each area, in addition to the focus positions. You can change such settings during the course of a time lapse imaging.

Cell Voyager 1000 Applications

Developmental biology

Long-term, 4D timelapse imaging

Following the injection of mouse embryos with mRNA, nearly 25,000 multicolor and multilayer confocal images of the embryos were acquired over 60 hour period as they developed to the blastocysts stage.Thereafter, they were transferred to a recipient mouse that gave birth to healthy pups, each of which developed normally and had full reproductive capability.This is firm evidence that long-term, multi-dimensional confocal imaging with CV1000 causes no harm to a delicate specimen such as an early stage embryo.

DATA:Kazuo Yamagata, PhD, Wakayama Lab.
(Laboratory for Genomic Reprogramming),
Center for Developmental Biology, RIKEN

Green FSpindle
 EGFP- α -tubulin
Red Nucleus H2B-mRFP1

Developmental biology

Wide-area imaging of primordial germ cells

The process to form colonies of EG cells(a kind of iPS cell)from primordial germ cells expressing GFP of 12.5 days embryo of TG mouse was imaged for a long-time at the whole area of a culture dish(625fields). As a result of 5 days imaging, colonies of EG cells were formed as frequently as was formed when the cells were cultured in a CO₂ incubator. With he CV1000, you can record whole area quite at ease when you don't know where to find the target, and can discover what happened from the acquired data.

DATA Yasuhisa Matsui, PhD, Cell Resource Center, Institute of Development, Aging and Cancer, Tohoku University.

GreenFMembrane EGFP

Cancer application

High-speed 4D imaging

Floating 293F cells were transfected with eGFP by using NeoFection, a transfection accelerating agent made by ASTEC.The cells were shake-cultured over night. As a result of time lapse imaging, active movement of cells expressing eGFP inside the floating cell clusters, and structural changes in the cell wall such as ruffing were clearly observed.

DATA  ASTEC CO,LTD.

Green Membrane EGFP

Plant application

High-speed multi-field imaging

Changes in the shape of a vacuolar membrane during the germination process were continuously recorded. High-speed and multi-point time lapse imaging with the CV1000 allows accurate and high-resolution tracking of rapid changes in living organisms, something that has proven quite difficult with conventional imaging systems. By selecting the appropriate filter and pinhole size, thick samples and auto fluorescent plant cells can be clearly observed with the CV1000.

DATA Chieko Saito, PhD, Senior Research Scientist, Live=cell Molecular Imaging research Team, Extreme Photonics Research Group,RIKEN

Green Vacuolar Membrane
Vam3-GFP